Polymerase chain reaction (PCR) and subsequent fragment analysis of paired normal and tumor tissue is used to determine microsatellite instability (MSI). Results are reported as MSI-H (at least two markers are unstable) or MSS (less than two markers are unstable).

The Therascreen® KRAS RGQ PCR Kit is a real-time qualitative PCR assay used on the Roto-Gene® Q MDx instrument for the detection of seven somatic mutations in codons 12 and 13 (exon 2) of the human KRAS oncogene, using DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue.

There are key genes recurrently mutated in colorectal cancer. Genomic DNA is isolated from formalin fixed paraffin embedded tissue and the DNA sequence of targeted regions of the BRAF, KRAS, and NRAS genes is determined using next-generation sequencing (NGS) technology.

The cobas 4800 BRAF V600 Mutation Test is a real-time PCR assay intended for the qualitative detection of the BRAF V600E mutation in DNA extracted from formalin fixed, paraffin-embedded human tumor tissue.