Cytogenetic abnormalities commonly associated with chronic lymphocytic leukemia (CLL) are detected by fluorescence in situ hybridization (FISH). Probes for IGH/CCND1 t(11;14), ATM (11q22.3), Cen 12 (+12), 13q (13q-/-13), TP53 (17p13.1) are included in the CLL FISH Profile (Enriched). B-cells are enriched from patient’s specimen using immunomagnetic enrichment technology with CD19/CD20 antibodies.

Cytogenetic abnormalities commonly associated with chronic lymphocytic leukemia (CLL) are detected by fluorescence in situ hybridization (FISH). Probes for IGH/CCND1 t(11;14), ATM (11q22.3), Cen 12 (+12), 13q (13q-/-13), TP53 (17p13.1) are included in the CLL FISH Profile.

Cytogenetic abnormalities commonly associated with high-grade B-cell non-Hodgkin lymphomas (NHL) are detected by fluorescence in situ hybridization (FISH). Probes for BCL6 (3q27.3), IRF4-DUSP22 (6p25.3), MYC (8q24.21) and IGH/BCL2 t(14;18) are included in the B-NHL High Grade FISH Profile.

Cytogenetic abnormalities commonly associated with acute myeloid leukemia (AML) are detected by fluorescence in situ hybridization (FISH). Probes for MECOM (3q26.2), DEK/NUP214 t(6;9), RUNX1T1/RUNX1 t(8;21), KMT2A (MLL)(11q23.3), PML/RARA t(15;17) and CBFb/MYH11 inv(16) are included in the AML FISH Profile.

Cytogenetic abnormalities commonly associated with acute lymphoblastic leukemia (ALL) are detected by fluorescence in situ hybridization (FISH). Probes PDGFRb (5q32), BCR/ABL1-ASS1 t(9;22), JAK2 (9p24.1), EPOR (19p13.2) and CRLF2 (Xp22.33/Yp11.32) are included in the ALL FISH (Ph-like) Profile.

Cytogenetic abnormalities commonly associated with pediatric acute lymphoblastic leukemia (ALL) are detected by fluorescence in situ hybridization (FISH). Probes for Cen 4, Cen 10 (+4/+10), BCR/ABL1-ASS1 t(9;22), KMT2A (MLL)(11q23.3), ETV6/RUNX1 t(12;21)/(iAMP21) and IGH (14q32.3) are included for pediatric cases of ALL.

Detects FLT3 and NPM1 mutations, which are commonly found in acute myeloid leukemia (AML). FLT3 ITD and TKD regions are analyzed by polymerase chain reaction (PCR) and fluorescent fragment size analysis. The signal ratio of the FLT3 ITD region compares the signal intensity of the mutation to the wild-type. Exon 12 of NPM1 is analyzed by PCR and fluorescent fragment size analysis to detect small insertion mutations specific to AML.

Internal Tandem Duplication (ITD) and Tyrosine Kinase Domain (TKD). FLT3 ITD and TKD regions are analyzed by polymerase chain reaction (PCR) and fluorescent fragment size analysis. The signal ratio of the FLT3 ITD region compares the signal intensity of the mutation to the wild-type.

Exon 12 of NPM1 is analyzed by polymerase chain reaction (PCR) and fluorescent fragment size analysis to detect small insertion mutations specific to AML.