Detection of mutations in key genes recurrently mutated in myeloid malignancies. Genomic DNA is isolated from bone marrow aspirates or peripheral blood and the DNA sequence of targeted regions of the ASXL1, BCOR, BRAF, CALR, CBL, CEBPA, CSF3R, DDX41, DNMT3A, ETNK1, ETV6, EZH2, GATA2, GNAS, GNB1, IDH1, IDH2, JAK2, KIT, KRAS, MPL, NF1, NPM1, NRAS, PDGFRA, PHF6, PPM1D, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, TET2, TP53, U2AF1, WT1, ZRSR2 genes is determined using next-generation sequencing (NGS) technology.

Detects mutations in key genes recurrently mutated in myeloproliferative neoplasms (MPN). DNA sequence of targeted regions of the CALR, CSF3R, JAK2, MPL and SETBP1 genes is determined using next generation sequencing technology.

Detects the JAK2 V617F mutation, which is commonly found in myeloproliferative neoplasms (MPN). DNA is isolated and subjected to allele-specific polymerase chain reaction (PCR) amplification.

Detects JAK2 exons 12 and 13 mutations, which are found a subset of myeloproliferative neoplasms (MPN). DNA sequences of JAK2 exons 12 and 13 are determined using next-generation sequencing technology.

Detects CALR mutations, which are found in a subset of myeloproliferative neoplasms (MPN). DNA sequence of exon 9 of the CALR gene is determined using amplicon-based, next-generation sequencing (NGS) technology.

Cytogenetic abnormalities commonly associated with myeloproliferative neoplasms (MPN) are detected by fluorescence in situ hybridization (FISH). Probes for CKS1B-CDKN2C (1p32.3/1q21.3), 5q (5q-/-5/+5), 7q (7q-/-7), Cen 8 (+8), BCR/ABL1-ASS1 t(9:22), 13q (13q-/-13), 20q (20q-) are included in the MPN FISH Profile