Hematologic Diseases

Cytogenetic abnormalities commonly associated with pediatric acute lymphoblastic leukemia (ALL) are detected by fluorescence in situ hybridization (FISH). Probes for Cen 4, Cen 10 (+4/+10), BCR/ABL1-ASS1 t(9;22), KMT2A (MLL)(11q23.3), ETV6/RUNX1 t(12;21)/(iAMP21) and IGH (14q32.3) are included for pediatric cases of ALL.

disease state indication(s)
Acute lymphoblastic leukemia (ALL), B Lymphoblastic Leukemia/Lymphoma

clinical use
The ALL FISH Profile (Pediatric) detects cytogenetic abnormalities commonly associated with pediatric acute lymphoblastic leukemia (ALL). Identification of specific abnormalities define various subtypes of ALL that have different prognosis and responsiveness to specific therapies.

methodology/product platform
Fluorescence in situ hybridization (FISH)

specimen type and requirements
Peripheral blood: 5-6 mL in sodium heparin (green-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube.
Lymphoid tissue: Transport in RPMI.
FFPE tissue: no decalcification.
Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.
DO NOT FREEZE.

Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will prioritize testing in the following order: BCR/ABL1-ASS1, KMT2A (MLL)(11q23.3), ETV6/RUNX1/(iAMP21), Cen 4, Cen 10, and IGH, unless directed otherwise by the client.

turnaround time
Tech-only (TC): 3 days
Global (TC & PC): 5 days

cpt code(s)
88377 (x5)

medicare moldx cpt code
Not applicable

regulatory classification
Laboratory developed test (LDT)

ordering option
Global (TC & PC) or Tech-only (TC)