Hematologic Diseases

Cytogenetic abnormalities commonly associated with acute lymphoblastic leukemia (ALL) are detected by fluorescence in situ hybridization (FISH). Probes PDGFRb (5q32), BCR/ABL1-ASS1 t(9;22), JAK2 (9p24.1), EPOR (19p13.2) and CRLF2 (Xp22.33/Yp11.32) are included in the ALL FISH (Ph-like) Profile.

disease state indication(s)
Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL), B lymphoblastic leukemia/lymphoma

clinical use
The ALL FISH Profile (Ph-like) detects cytogenetic abnormalities commonly associated with Philadelphia chromosome (Ph-like) acute lymphoblastic leukemia (ALL). Patients with Ph-like B-ALL are associated with an unfavorable prognosis, but may potentially be amenable to inhibition with select tyrosine kinase inhibitors.

methodology/product platform
Fluorescence in situ hybridization (FISH)

specimen type and requirements
Peripheral blood: 3-5 mL in sodium heparin (green-top) tube or EDTA (purple-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube or EDTA (purple-top) tube.
Lymphoid tissue: Transport in RPMI.
FFPE tissue: No decalcification. Two (2) x four (4) um thick slides for each probe ordered.
Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.

Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will prioritize testing in the following order: BCR/ABL1, CRLF2, JAK2, EPOR, and PDGFRβ, unless directed otherwise by the client.

turnaround time
Tech-only (TC): 3 days
Global (TC & PC): 5 days

cpt code(s)
88377 (x5)

medicare moldx cpt code
Not applicable

regulatory classification
Laboratory developed test (LDT)

ordering option
Global (TC & PC) or Tech-only (TC)