Hematologic Diseases

Cytogenetic abnormalities commonly associated with high-grade B-cell non-Hodgkin lymphomas (NHL) are detected by fluorescence in situ hybridization (FISH). Probes for BCL6 (3q27.3), IRF4-DUSP22 (6p25.3), MYC (8q24.21), IGH/BCL2 t(14;18)) are included in the B-NHL High-Grade FISH Profile (Enriched). B-cells are enriched from patient’s specimen using immunomagnetic enrichment technology with CD19/CD20 antibodies.

disease state indication(s)
B-cell non-Hodgkin lymphomas (NHL), follicular lymphoma (FL), mantle cell lymphoma (MCL), marginal zone lymphoma (MZL), lymphoplasmacytic lymphoma (LPL), diffuse large B-cell lymphoma (DLBCL), Burkitt lymphoma (BL), and double-hit lymphoma (DHL)

clinical use
The B-NHL High-Grade FISH Profile (Enriched) detects cytogenetic abnormalities commonly associated with high-grade B-cell non-Hodgkin lymphomas (NHL), including diffuse large B-cell lymphoma (DLBCL), Burkitt lymphoma (BL), double-hit lymphoma (DHL), and triple-hit lymphoma (THL). FISH analysis on specimens enriched for B-cells can enhance the ability to detect abnormalities, particularly in cases with a low percentage of monoclonal B-cells.

methodology/product platform
Fluorescence in situ hybridization (FISH)

specimen type and requirements
Peripheral blood: 5-6 mL in sodium heparin (green-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube.
FFPE tissue: no decalcification (except for centromere as well as locus specific FISH probes).
Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.
DO NOT FREEZE.

Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will contact client to prioritize testing,

turnaround time
Tech-only (TC): 3 days
Global (TC & PC): 5 days

cpt code(s)
88377 (x6)
88112-TC (x1)

medicare moldx cpt code
Not applicable

regulatory classification
Laboratory developed test (LDT)

ordering option
Global (TC & PC) or Tech-only (TC)