Cytogenetic abnormalities commonly associated with high-grade B-cell non-Hodgkin lymphomas (NHL) are detected by fluorescence in situ hybridization (FISH). Probes for BCL6 (3q27.3), IRF4-DUSP22 (6p25.3), MYC (8q24.21), IGH/BCL2 t(14;18)) are included in the B-NHL – High Grade FISH Profile (Enriched). B-cells are enriched from patient’s specimen using immunomagnetic enrichment technology with CD19/CD20 antibodies.
disease state indication(s)
Diffuse large B-cell lymphoma (DLBCL), Burkitt lymphoma (BL), double-hit lymphoma (DHL), triple-hit lymphoma (THL)
The B-NHL - High Grade FISH Profile (Enriched) detects cytogenetic abnormalities commonly associated with high-grade B-cell non-Hodgkin lymphomas (NHL), including diffuse large B-cell lymphoma (DLBCL), Burkitt lymphoma (BL), double-hit lymphoma (DHL), and triple-hit lymphoma (THL). FISH analysis on specimens enriched for B-cells can enhance the ability to detect abnormalities, particularly in cases with a low percentage of monoclonal B-cells.
Fluorescence in situ hybridization (FISH)
specimen type and requirements
Peripheral blood: 5-6 mL in sodium heparin (green-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube.
Lymphoid tissue: Transport in RPMI.
FFPE tissue: no decalcification (except for centromere as well as locus specific FISH probes).
Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.
DO NOT FREEZE.
Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will prioritize testing in the following order: MYC, IGH/BCL2, BCL6, and IRF4-DUSP22, unless directed otherwise by the client.
Tech-only (TC): 3 days
Global (TC & PC): 5 days
medicare moldx cpt code
Laboratory developed test (LDT)
Global (TC & PC) or Tech-only (TC)