Cytogenetic abnormalities commonly associated with chronic myelogenous leukemia (CML) and CML in transformation are detected by fluorescence in situ hybridization (FISH). Probes for Cen 8 (+8), BCR/ABL1-ASS1 t(9;22), RARA (17q21.2)/(iso17q) are included in the CML FISH Profile.
disease state indication(s)
Chronic myelogenous leukemia (CML)
The CML FISH Profile detects the t(9;22) BCR-ABL1 fusion gene, which is the hallmark finding in chronic myelogenous leukemia (CML), as well as additional cytogenetic abnormalities commonly associated with transformation.
Fluorescence in situ hybridization (FISH)
specimen type and requirements
Peripheral blood: 5-6 mL in sodium heparin (green-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube.
Bodily fluids: Bodily fluids for diagnostic testing of hematological cancers (subject to specimen viability). Preferred transport in equal parts RPMI.
Lymphoid tissue: Transport in RPMI.
FFPE tissue: no decalcification (except for centromere as well as locus specific FISH probes)
Note: Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.
DO NOT FREEZE.
Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will prioritize testing in the following order: BCR/ABL1-ASS1 t(9;22), Cen 8, and RARA/(iso17q), unless directed otherwise by the client.
Tech-only (TC): 3 days
Global (TC & PC): 5 days
medicare moldx cpt code
Laboratory developed test (LDT)
Global (TC & PC) or Tech-only (TC)