Hematologic Diseases

Cytogenetic abnormalities commonly associated with myeloproliferative neoplasms (MPN) are detected by fluorescence in situ hybridization (FISH). Probes for CKS1B-CDKN2C (1p32.3/1q21.3), 5q (5q-/-5/+5), 7q (7q-/-7), Cen 8 (+8), BCR/ABL1-ASS1 t(9:22), 13q (13q-/-13), 20q (20q-) are included in the MPN FISH Profile

disease state indication(s)
Myeloproliferative neoplasms (MPN), chronic myelogenous leukemia (CML), essential thrombocythemia (ET), primary myelofibrosis (PMF), polycythemia vera (PV)

clinical use
The MPN FISH Profile detects cytogenetic abnormalities commonly associated with myeloproliferative neoplasms (MPN), including chronic myelogenous leukemia (CML).

methodology/product platform
Fluorescence in situ hybridization (FISH)

specimen type and requirements
Peripheral blood: 5-6 mL in sodium heparin (green-top) tube.
Bone marrow: 2-3 mL in sodium heparin (green-top) tube.
Bodily fluids: Bodily fluids for diagnostic testing of hematological cancers (subject to specimen viability). Preferred transport in equal parts RPMI.
Lymphoid tissue: Transport in RPMI.
FFPE tissue, no decalcification (except for centromere as well as locus specific FISH probes).
Use refrigerated cold pack for transport. Make sure cold pack is not in direct contact with specimen.
DO NOT FREEZE.

Probe Prioritization: If samples are insufficient to complete the whole FISH panel, Genoptix will prioritize testing in the following order: BCR/ABL1-ASS1, 7q, 5q, Cen 8, 20q, 13q, and CKS1B-CDKN2C, unless directed otherwise by the client.

turnaround time
Tech-only (TC): 3 days
Global (TC & PC): 5 days

cpt code(s)
88368 (x1)
88369 (x1)
88377 (x5)

medicare moldx cpt code
Not applicable

regulatory classification
Laboratory developed test (LDT)

ordering option
Global (TC & PC) or Tech-only (TC)